What is the 10⁴ factor?

Each large square on a standard hemocytometer has a volume of 0.1 µL (10⁻⁴ mL). Multiplying by 10⁴ scales the average count to cells per milliliter.

How many squares should I count?

Counting four or five large squares (or all 25 small squares) increases accuracy. Always average the counts.

Can I use this for yeast or bacteria?

Yes, any cell type counted with a hemocytometer can use the same formula as long as you apply the appropriate dilution factor.

Hemocytometer Cell Counting Calculator

Audit: Needs review

Formula (LaTeX) + variables + units

This section shows the formulas used by the calculator engine, plus variable definitions and units.
Note: This page needs review to confirm formulas and sources.

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Variables and units

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Sources (authoritative):

Home — calcdomain.com · Accessed 2026-01-19
https://calcdomain.com/
Science — calcdomain.com · Accessed 2026-01-19
https://calcdomain.com/science

Changelog

Version: 0.1.0-draft
Last code update: 2026-01-19

0.1.0-draft · 2026-01-19

Initial audit spec draft generated from HTML extraction (review required).
Verify formulas match the calculator engine and convert any text-only formulas to LaTeX.
Confirm sources are authoritative and relevant to the calculator methodology.

Verification pending · Last code update: 2026-01-19