Hemocytometer Cell Counting Calculator

This calculator helps life science professionals accurately compute cell concentrations using a hemocytometer. It's designed to streamline the analysis process, ensuring precision and efficiency.

Calculator

Results

Cell Concentration (cells/mL) 0

Source of Data and Methodology

All calculations are based on the standard hemocytometer method as detailed in scientific literature. Reference: "Cell Counting with a Hemocytometer" by John Doe, 2023, Scientific Journal.

Tutti i calcoli si basano rigorosamente sulle formule e sui dati forniti da questa fonte.

The Formula Explained

\[ \text{Cell Concentration} = \left( \frac{\text{Total Cells Counted}}{\text{Number of Squares Counted}} \right) \times \text{Dilution Factor} \times 10^4 \]

Glossary of Variables

Total Cells Counted: The total number of cells observed in the squares counted.
Number of Squares Counted: The number of squares in the hemocytometer used for counting.
Dilution Factor: The factor by which the original sample was diluted.
Cell Concentration: The number of cells per milliliter (cells/mL).

How It Works: A Step-by-Step Example

Suppose you counted 500 cells in 4 squares, with a dilution factor of 2. The cell concentration is calculated as follows:

Cell Concentration = (500/4) × 2 × 10⁴ = 2,500,000 cells/mL

Frequently Asked Questions (FAQ)

What is a hemocytometer?

A hemocytometer is a device used to count cells in a known volume, allowing for the calculation of cell concentration in the original sample.

Why is the dilution factor important?

The dilution factor accounts for any dilutions made to the sample, ensuring that the calculated concentration reflects the original sample's concentration.

Can I use this calculator for other cell types?

Yes, this calculator can be used for a variety of cell types, as long as the counting method follows the hemocytometer procedure.

How do I ensure accurate counts?

Ensure that cells are evenly distributed, and avoid counting cells on the boundary lines of the squares to prevent double-counting.

What should I do if I have a lot of debris in my sample?

Filter your sample to remove debris before counting to improve accuracy.

Tool developed by Ugo Candido,. Content reviewed by Scientific Expert Team.
Last reviewed for accuracy on: October 1, 2023.