What is a restriction enzyme digest?

It is a process in molecular biology where DNA is cut at specific sites by restriction enzymes.

How do I use this calculator?

Enter the DNA sequence and select the restriction enzyme to see possible fragment sizes.

Restriction Enzyme Digest Calculator

This calculator helps molecular biologists determine the fragment sizes produced by restriction enzyme digestion of DNA.

Results

Data Source and Methodology

All calculations are based on standard restriction enzyme recognition sequences as provided by New England Biolabs. All calculations are strictly based on the formulas and data provided by this source.

The Formula Explained

The formula used for calculating the fragment sizes is based on the recognition sequence of the chosen enzyme and the cut sites within the DNA sequence.

Glossary of Variables

DNA Sequence: The sequence of nucleotides that you want to digest.
Restriction Enzyme: The enzyme used to cut the DNA at specific recognition sites.

How It Works: A Step-by-Step Example

For example, using the DNA sequence 'GAATTCGAATTC' with the EcoRI enzyme, the calculator will identify the cut sites and display the resulting fragments.

Frequently Asked Questions (FAQ)

What is a restriction enzyme?

Restriction enzymes are proteins used to cut DNA at specific sequences.

How do I know which enzyme to use?

The choice of enzyme depends on the sequence you want to analyze and the cut pattern you need.

Can I input a sequence longer than 1000 base pairs?

Yes, but longer sequences may take more time to process.

Why are no fragments appearing in the results?

Ensure your DNA sequence contains the recognition site of the selected enzyme.

How can I export the results?

Use the browser's print feature to save the results as a PDF.

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