Data Source and Methodology
All calculations are based on standard formulas for serial dilutions as per scientific protocols. For further reading, refer to our authoritative resources at AAT Bioquest. All calculations adhere strictly to the formulas and data provided by this source.
The Formula Explained
Final Concentration = Initial Concentration / (Dilution Factor ^ Number of Dilutions)
Glossary of Variables
- Initial Concentration: The starting concentration of a solution.
- Dilution Factor: The factor by which the solution is diluted each step.
- Number of Dilutions: The number of times the solution is diluted.
- Final Concentration: The resulting concentration after all dilutions.
How It Works: A Step-by-Step Example
Consider an initial concentration of 100 µM, a dilution factor of 10, and 3 dilution steps. Using the formula, the final concentration is calculated as 100 / (10^3) = 0.1 µM.
Frequently Asked Questions (FAQ)
What is a serial dilution?
A serial dilution is a stepwise dilution of a substance in solution, usually by a consistent factor with each step.
Why are serial dilutions used?
Serial dilutions are used to create solutions of decreasing concentration, which is useful in experiments to determine the concentration of a substance in a sample.
How do I choose a dilution factor?
The dilution factor depends on the desired final concentration and the initial concentration. It is often a power of 10.
How accurate is this calculator?
This calculator provides accurate results based on the inputs and standard dilution formulas. Accuracy depends on the precision of input values.
Can I use this calculator for different units?
Yes, as long as all inputs are in consistent units, the calculator can be used for any unit of concentration.